EN
Single Cell Sequencng
The single-cell DNBelab C4 technology platform, based on the high-throughput single-cell library preparation system DNBelab C4, combined with the independently developed ultra-high-throughput sequencing system DNBSEQ, can achieve sequencing data output on a scale of millions of cells in a single run. It can be used for detecting scRNA-seq and scATAC-seq, significantly reducing library preparation costs while generating a comprehensive cellular atlas. This platform currently represents a full-process technology platform capable of high-throughput, low-cost, and one-stop single-cell omics research.
DNBelab C4 system
Technical development
Since the advent of single-cell sequencing technology in 2009, it has undergone over a decade of development and optimization (Figure 2b). Various single-cell isolation and sequencing techniques for different omics have also been developed (Figure 2a). Among these, the most mature and widely used technology is single-cell transcriptome sequencing (single-cell RNA-seq, scRNA-seq). By 2015, scRNA-seq technology had achieved ultra-high throughput levels, capable of sequencing thousands of cells in a single run on a droplet microfluidics platform (3’scRNA-seq), enabling rapid sequencing of a large number of cells.
Applications
At present, research articles in the field of single-cell omics can be roughly divided into the following types based on their application directions.
Disease
Growth and development
Life evolution
Research methods
Technical Principles
Technical Procedure
Library Construction Procedure
Sequencing Strategy
Data Analysis
Advantages
